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How To Protocols

The better epitope- the less animals you have to immunize

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There is always a question of how many animals you have to immunize. Standard - 2-3 rabbits and 4 mice per antigen is more or less accepted numbers to start with. However, you can predict with a relatively high accuracy how many animal a particular antigen requires. It's defined by antigen's antigenicity index.

Let's look at the already disussed example of four peptide epitopes from erbB2 used for immunization

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